Was gehört zum Protoplast?
Das Innere einer Pflanzenzelle – also Zellkern, Cytoplasma und Zellmembran – wird als Protoplast bezeichnet. Durch Enzyme (Cellulasen, Pektinasen) können die pflanzlichen Zellwände aufgelöst und so gezielt Protoplasten gewonnen werden. Nach Entfernen der Zellwand nehmen Protoplasten eine kugelige Gestalt an.
Was ist eine Turgeszente Zelle?
turgescens = strotzend; Subst. Turgeszenz], Bezeichnung für mit Flüssigkeit prall gefüllte und dadurch „gespannte“ (unter Druck stehende) Zellen und Gewebe.
What are the two methods of protoplast fusion?
The following points highlight the two methods of protoplast fusion. They are: (1) Spontaneous Fusion and (2) Induced Fusion. Method # 1. Spontaneous Fusion: Protoplasts during isolation often fuse spontaneously and this phenomenon is called spontaneous fusion.
How do you Fuse protoplasts?
Electrical fusionIf Protoplasts are placed into a small culture vessel containing electrodes and apotential difference is applied, then the protoplasts will line up between the electrodes.If now an extremely short, electric shock is applied, protoplasts can be induced tofuse. 17. Protoplast Fusion Induced by the Application of Electric Shock
What are chemical fusogens and how do they affect protoplasts?
Chemical fusogens cause the isolated protoplasts to adhere to one another and leads to tight agglutination followed by fusion of protoplast (Fig. 6.14). The adhesion of isolated protoplast takes place either due to reduction of negative charges of protoplast or due to attraction of protoplast by electrostatic forces caused by chemical fusogens.
How are protoplasts made?
Protoplasts can be made bydegrading cell walls with a mixture of the appropriate polysaccharide-degradingenzymes:•During and subsequent to digestion of the cell wall, the protoplast becomes verysensitive to osmotic stress.